In a club that opened in 1933 but didn't admit its first women members until 2012, LPGA legends Pak Se Ri, Annika Sorenstam, Lorena Ochoa and Nancy Lopez hit ceremonial shots off the first tee to launch the historic final round of the inaugural Augusta National Women's Amateur.
England's Meghan McClaren is the leading female in the field after she opened her account with a seven-under-par 65.
They were among six players who finished under par.
Women's world amateur number one Kupcho, the reigning U.S. college champion, and Fassi, last year's USA women's college golfer of the year, staged a dramatic duel in a historic moment.
The 12th hole is shown at "Amen Corner", where golfers turn the corner on the 11th, 12th and 13th holes, at the Masters golf tournament at the Augusta National Golf Club in Augusta, Georgia, April 6, 2018.
Australian Julienne Soo missed the cut - but she will still play a practice round at Augusta National tomorrow. "I started out well but had a couple of bad holes on the back nine". The Wake Forest University senior led after 36 holes on 5-under par 139 with Mexico's Maria Fassi, a University of Arkansas senior, one stroke behind.
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In a dramatic battle between two 21-year-old United States collegiate stars, Kupcho had three birdies and an eagle in the last six holes in firing a five-under par 67 to finish 54 holes on 10-under 206 for a four-stroke victory over Fassi, who fired her third consecutive 70. On Thursday, she eagled the toughest hole on the golf course - the par-5 14th where she chipped in from 40 yards - and made an additional five birdies, but also mixed in five bogeys.
Though he hasn't been very good in five tournaments this year, Woods has been in the hunt in the last two majors he's played in and has a history of playing well at Augusta.
"It's just like it's hard to explain in words because I never expected it to be that special" said Ana Belac.
Earlier in the round, 21-year-old Kupcho fought a migraine that resulted in blurry vision, but she battled back to record a five-under-par 67 - fittingly, the lowest round of the championship - to win by four strokes on 10-under-par 206.
The first competitive round played by women at Augusta National attracted a large crowd but it was not the typical gallery seen at the Masters.
The Augusta National Women's Amateur has its own logo and even its own theme music for the broadcast. The final three hours of play will air on NBC sports starting at noon. "So I think we'll make a lot of birdies, and it will be pretty fun to watch us".